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MALDI-TOF-MS Analysis of Protein and DNA

Institute of Biochemistry, University of Erlangen-Nürnberg, Erlangen, Germany

Andreas Humeny

Institute of Biochemistry, University of Erlangen-Nürnberg, Erlangen, Germany, andreas.humeny{at}biochem.uni-erlangen.de

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) has become a powerful and widespread analytical tool in all fields of life science. The wide mass range (1-300 kDa), high accuracy, and sensitivity make it a superior method for analysis of all kinds of biomolecules including proteins, nucleic acids, and carbohydrates. In combination with 2D-gelelectrophoresis, MALDI-TOF-MS is particularly suitable for the identification of protein spots via mass fingerprint or microsequencing. Furthermore, the method allows a detailed analysis of posttranslational protein modifications. Recently, the method was also successfully applied to DNA sequencing as well as screening for mutations. Thus, high-throughput genotyping of single nucleotide polymorphisms has the potential to become a routine method for both laboratory and clinical applications.

Key Words: MALDI • Mass spectrometry • Neurochemistry • Genetic analysis • Protein analysis

The Neuroscientist, Vol. 7, No. 1, 6-12 (2001)
DOI: 10.1177/107385840100700104


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